Figure 3: CaFEN1 and CaFEN12 deletants of C. albicans are impaired in cell wall integrity. | Scientific Reports

Figure 3: CaFEN1 and CaFEN12 deletants of C. albicans are impaired in cell wall integrity.

From: Critical role for CaFEN1 and CaFEN12 of Candida albicans in cell wall integrity and biofilm formation

Figure 3

(a) C. albicans strains deleted in CaFEN1 and CaFEN12 genes are hypersensitive to CFW. Cells of the parent (SN95) and single as well as double deletant strains of CaFEN1 and CaFEN12 genes were spotted onto synthetic complete agar plates with indicated concentration CFW and incubated at 30 °C for 2 days. (b) Chitin level of single and double deletants of CaFEN1 and CaFEN12 genes. After CFW staining images were captured under identical conditions with a fluorescence microscope using 100× objective lens. (c) Zymolyase sensitivity, determined as described in Fig. 2b. (d) SDS sensitivity. Cells of the parent and deletants were incubated with indicated concentration of SDS as mentioned in Fig. 2c.

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