Figure 7: FSP1 expression during microvessel formation.

(a,b) Orthogonal views of the cross sections of the Matrigel plug with GFP⁺ cells, which were immunostained by the antibodies against ACTA2 and FSP1. Scale bar, 10 μm. Arrowhead, GFP⁺/ACTA2⁺/FSP1⁺ cells. Arrow, GFP⁺/ACTA2⁺ cells. (c) Sox10⁺ stem cells were cultured in the medium with 10% FBS and 10 ng/ml TGFβ1 for 3 and 14 days, and immunostained by antibodies against ACTA2 and FSP1. Cell nuclei were stained by DAPI. Scale bar, 100 μm. Hollow arrowhead, FSP1⁺ cells. Arrowhead, ACTA2⁺/FSP1⁺ cells. Arrow, ACTA2⁺ cells. (d) Percentages of different cell populations were quantified. Mean ± SD of n = 3 independent experiments of n = 12 fields for each group. Student’s t-test was performed to analyze significant differences between groups. **P < 0.01.