Figure 4: GLA-LSQ adjuvant promotes rapid expansion of Tfh cells and plasmablasts after boost immunization (Day 33). | Scientific Reports

Figure 4: GLA-LSQ adjuvant promotes rapid expansion of Tfh cells and plasmablasts after boost immunization (Day 33).

From: Adjuvant and carrier protein-dependent T-cell priming promotes a robust antibody response against the Plasmodium falciparum Pfs25 vaccine candidate

Figure 4

Mice were immunized i.m. with 1 μg Pfs25-EPA or Pfs25-TT in Alhydrogel or GLA-LSQ on day 0 and day 28. Spleens and inguinal LNs were harvested on day 33 post-immunization and processed for flow cytometry. (a) Representative flow cytometry plots showing, within the activated CD44hiFoxp3−CD4+ T cell population, the frequency of ICOS+PD-1+ cells (left column) and within this population the frequency of Bcl6+CXCR5+ cells (right column) in the inguinal LN. (b) Histogram showing the frequency of ICOS+PD-1+CXCR5+Bcl6+ Tfh cells within the CD44hiFoxp3−CD4+ T cell population in the inguinal LN. (c) Histogram showing the ratio of Foxp3− (Tfh cells) versus Foxp3+ (Tfr cells) within the CXCR5+Bcl6+ CD4+ T cell population in the inguinal LN. (d) Expansion of Pfs25+IgG+ specific plasmablasts in the spleens of immunized mice on day 33. IC denotes intracellular staining with fluorescent Pfs25 or with an anti-IgG antibody. (e) Number of Pfs25+IgG+ splenic plasmablasts on day 33 calculated via flow cytometry. Shown is the mean ± SEM, n = 10 mice per group. Data are pooled from 2 similar experiments. (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; One-way ANOVAs with Tukey post-tests).

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