Figure 5: Adjuvant-dependent induction of functional antibodies with transmission-reducing activity. | Scientific Reports

Figure 5: Adjuvant-dependent induction of functional antibodies with transmission-reducing activity.

From: Adjuvant and carrier protein-dependent T-cell priming promotes a robust antibody response against the Plasmodium falciparum Pfs25 vaccine candidate

Figure 5

Mice were immunized i.m. with 1 μg Pfs25-EPA or Pfs25-TT in Alhydrogel or GLA-LSQ on day 0 and day 28. Sera were collected on day 42 or 250 post-immunization, pooled from 10 mice per group, and diluted (1:8) with naïve sera. Diluted sera and P. falciparum NF54 gametocytes were fed to Anopheles stephensi mosquitoes and the oocyst numbers in mosquito midguts were counted as an indication of infectivity. (a) Number of oocysts per mosquito as determined by membrane feeding assays (MFA). Pre-immune sera are from naïve controls. Shown is the mean ± SEM, n = 22–24 dissected mosquitoes per group. Infection prevalence (number of mosquitoes infected/number of mosquitoes dissected) is expressed as a percentage beneath the x-axis. (b) Comparison of transmission-reducing activity of sera relative to ELISA titers. Data shown are mean ELISA titers versus percentage of oocyst reduction as compared to control. The black line shows the nonlinear regression fit of the data. Data are from 1 experiment and are representative of 2 experiments conducted with 2 separate feeds. (*P < 0.05, **P < 0.01, ***P < 0.001; One-way ANOVA with Tukey post-tests).

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