Figure 5: Fewer mature sperm in the testes of ERR knockdown males dispersed all along the testicular tract. | Scientific Reports

Figure 5: Fewer mature sperm in the testes of ERR knockdown males dispersed all along the testicular tract.

From: Estrogen related receptor is required for the testicular development and for the normal sperm axoneme/mitochondrial derivatives in Drosophila males

Figure 5

To determine if the impact of ERR on molecular players associated with spermatogenesis is reflected at the phenotypic level, we analyzed Protamine incorporation into sperm chromatin in control/knockdown males carrying ProtamineB-EGFP and counted the number of mature sperm. Both control and knockdown testes/seminal vesicles displayed sperm labeled with GFP (Green, Panels A and B) suggesting normal protamine incorporation. Further, control males (Panel A) have mature sperm in distended seminal vesicle (SV; indicated by white arrow). However, knockdown males had sperm (in green) scattered all along the globular testicular mass (Panel B, ERR-TRiP, labeled as T). The number of mature sperm in the SV (Panel C) of ERR knockdown males were significantly reduced (**pā€‰<ā€‰0.01). All these experiments were carried out twice and sperm were counted in tissues twice with a repeatability of index of 92%.

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