Figure 3 | Scientific Reports

Figure 3

From: Tryptophan and Non-Tryptophan Fluorescence of the Eye Lens Proteins Provides Diagnostics of Cataract at the Molecular Level

Figure 3

Fluorescence spectral and lifetime parameters of Trp, its derivatives and ArpP (A). A simulated emission spectrum generated from emission spectra of Trp and the OH-Trp, ArgP, NFK, Kyn and OH-Kyn in the 5:37:11:32:11:5 proportion (B) and spectral dependence of average fluorescence lifetime (inset). A typical example of fluorescence lifetime responses of the insoluble fraction of an emulsified donor eye lens (NC++) measured across the emission spectrum in the 350–550 nm range upon 317 nm excitation (C). Steady-state emission spectrum of this sample upon 317 nm excitation (D) and a spectral dependence of averaged lifetime calculated from the data shown in the left panel (inset).

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