Figure 4
Absorption spectra of AAWAA before (black) and after (red) UV irradiation (A). Emission spectra of AAWAA before UV irradiation upon 280 nm excitation (dash black), and after UV irradiation upon 280 nm (blue), 300 nm (green) 320 nm (red), 350 nm (cyan), 400 nm (magenta), 450 nm (yellow) and 500 nm (black) excitation (B). Normalised emission spectra (inset). Decomposition of the emission spectrum of Fr5 measured upon 372 nm excitation (black) using spectra associated with τ1,τ2 and τ3 (blue circles) and τ4 (red circles) lifetime components. The parameters were calculated by evaluating fluorescence time-responses of Fr5 in the 400–600 nm range by a 4-exponential model with “linked” lifetime parameters. (C) Blue-shifted by 5 nm fluorescence spectra of NFK (blue) and fluorescence spectrum of Kyn (red) in PBS.
