Figure 5
Fluorescence spectra of the soluble (A) and insoluble (B) fractions of emulsified donor eye lens proteins measures upon 310 nm excitation. The spectra were normalised on the emission spectrum of Trp (black) measured in samples of porcine eye lens proteins solubilised either in PBS (left panel, black) or in PBS/7 M urea buffer (right panel, black) upon 310 nm excitation. (C) Decomposition of the emission spectrum (excitation 310 nm) of the insoluble fraction of an emulsified cataractous sample (NC++) (black) over fluorescence spectra of NATA (1), AA-OH-Trp-AA (2), ArgP (3), AA-Kyn-AA (4), AA-Kyn-AA (5) and AA-OH-Kyn-AA (6) (blue) and corresponding fit-function parameters: C1 = 0.84, C2 = 0.21, C3 = 0.20, C4 = 0.46, C5 = 0.05, C6 = 0.08. Residuals function (bottom panel). The spectral decomposition was carried out using equation (4). Correlation of cataract grade with the normalised cumulative fraction (C2 + C3 + C4)/C1 for 21 emulsified eye lens samples (D).
