Figure 6
Decomposition of emission spectra (excitation 317 nm) of a 30 (A) and 80 years old (B) donor lenses over fluorescence spectra of NATA (1), AA-OH-Trp-AA (2), ArgP (3), AA-Kyn-AA (4), AA-Kyn-AA (5n) and AA-OH-Kyn-AA (6). Fit-functions (blue) with C1 = 0.74, C2 = 0.14 C3 = 0.14, C4 = 0.84, C5 = 0.03 C6 = 0.12 (panel A) and C1 = 0.42, C2 = 0.12, C3 = 0.39, C4 = 0.72, C5 = 0.00, C6 = 0.09 (panel B). Residuals functions (A,B bottom panels). The spectral decompositions were carried out using equation. (4). Correlation of age with normalised cumulative fraction (C2 + C3 + C4)/C1 for 14 donor lenses with age related nuclear cataracts (C).
