Figure 4: Tan IIA inhibited apoptosis pathway by immunohistochemistry and Western blot both in vivo and in vitro. | Scientific Reports

Figure 4: Tan IIA inhibited apoptosis pathway by immunohistochemistry and Western blot both in vivo and in vitro.

From: Tanshinone IIA induces intrinsic apoptosis in osteosarcoma cells both in vivo and in vitro associated with mitochondrial dysfunction

Figure 4

The number of positive cells of caspase 3, 8 and 9 (a) by IHC and the protein levels of caspase 3, 8 and 9 (b) by Western blot from tumor tissue in Tan IIA treatment group was more significantly enhanced than those in control group. 143B cells were treated with the indicated concentrations of Tan IIA for 24 hours. Cell lysates were subjected to Western blot analysis using antibodies including caspase-3, caspase 8, caspase 9 and β-actin antibodies. (c) All of caspase 3, 8, and 9 were activated by Tan IIA in 143B cells to trigger apoptosis. The bar value is the mean ± SD of three independent experiments in duplicate. An asterisk demonstrates the significant difference (p < 0.05) between control and Tan IIA-treated groups. The Western blot data from cell culture represent one of the three independent experiments.

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