Figure 5: The Bcl-2 family of proteins is critical for the intrinsic mitochondrial apoptotic pathway. | Scientific Reports

Figure 5: The Bcl-2 family of proteins is critical for the intrinsic mitochondrial apoptotic pathway.

From: Tanshinone IIA induces intrinsic apoptosis in osteosarcoma cells both in vivo and in vitro associated with mitochondrial dysfunction

Figure 5

They comprise anti-apoptotic (Bcl-2) and pro-apoptotic (including Bad, Bax and Bak) members that function coordinately to control caspase-dependent and -independent cell death. IHC (a) and Western blots (b) were shown that Bcl-2 was down regulated, on contrary, Bad, Bax and Bak were up regulated significantly in Tan IIA treatment group in comparison with those in none-treated groups. 143B cells were treated with the indicated concentrations of Tan IIA for 24 hours. Cell lysates were subjected to Western blot analysis using antibodies including Bcl-2, Bad, Bax, Bak and β-actin antibodies. (c) Tan IIA treatment led to the up regulation of pro-apoptotic protein expression such as Bad, Bax and Bak, while apoptotic protein including Bcl-2 was down regulated in a dose-dependent manner. (d) Bax and Bcl-2 ratio was increased. The bar value is the mean ± SD of three independent experiments in duplicate. An asterisk demonstrates the significant difference (p < 0.05) between control and Tan IIA-treated groups. The Western blot data from cell culture represent one of the three independent experiments.

Back to article page