Figure 6: Mitochondrial dynamic changes of morphology and apoptosis in osteosarcoma 143B cells by Tan IIA. | Scientific Reports

Figure 6: Mitochondrial dynamic changes of morphology and apoptosis in osteosarcoma 143B cells by Tan IIA.

From: Tanshinone IIA induces intrinsic apoptosis in osteosarcoma cells both in vivo and in vitro associated with mitochondrial dysfunction

Figure 6

Cells treated with 10µM Tan IIA for 24 hours were stained with (a) Mito-Tracker red and (b) JC-1 to demonstrate mitochondria in live cells. DAPI staining was used to detect all nuclei and visualized under a confocal microscope (200 ×). Tan IIA fragmented the mitochondrial network in the osteosarcoma 143B cells. Annexin V-FITC/propidium iodide staining (c) and TUNEL assay (d) was also performed to determine the level of apoptosis. There was significant increase in apoptotic levels in 143B cells with the treatment of Tan IIA. The bar value is the mean ± SD of three independent experiments in duplicate. Asterisks mean statistical significance in comparison with the vehicle control (P < 0.05).

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