Figure 1: EIF3a showed high expression in a cisplatin sensitive cell line. | Scientific Reports

Figure 1: EIF3a showed high expression in a cisplatin sensitive cell line.

From: MiR-488 inhibits proliferation and cisplatin sensibility in non-small-cell lung cancer (NSCLC) cells by activating the eIF3a-mediated NER signaling pathway

Figure 1

(a) Cells were treated with increasing concentrations of cisplatin (3 μM to 384 μM). Forty-eight hours later, cell viability was tested with MTS. The half maximal inhibitory concentration (IC50) was calculated from 3 independent experiments using GraphPad 5.0 software. The A549/DDP cell line showed a higher IC50 than the A549 cell line. (b) The relative expression of eIF3a mRNA in the A549/DDP cell line compared with the parental cell line was measured with qRT-PCR. (c) The eIF3a protein expression in the A549/DDP and A549 cell lines was determined by western blot. The bands cropped for the representative images are shown in Fig. 1c, and full-length blots are presented in Supplementary Fig. S3a. EIF3a was highly expressed in the cisplatin sensitive cell line (A549), and the data from three independent experiments is given as the mean ± SD (*P < 0.05).

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