Figure 3: MiRNA-488 directly targeted eIF3a.

(a) The pmiR-RB-REPORT™ (vector) containing wild-type or mutant eIF3a 3’UTRs was constructed using the matching seed sequence of miR-488 and eIF3a. (b) The relative luciferase activity of the indicated eIF3a reporter construct was analyzed in A549 cells. The Renilla luciferase activity of each sample was normalized to the firefly luciferase value and plotted as relative luciferase activity.