Figure 5: MiR-488 increased cisplatin resistance in three NSCLC cell lines. | Scientific Reports

Figure 5: MiR-488 increased cisplatin resistance in three NSCLC cell lines.

From: MiR-488 inhibits proliferation and cisplatin sensibility in non-small-cell lung cancer (NSCLC) cells by activating the eIF3a-mediated NER signaling pathway

Figure 5

(a) A549 and (d) H1299 cells transfected with miR-488 or NC were treated with different concentrations of DDP. Cell viability was tested with an MTS assay. The miR-488 group exhibited a higher IC50 of cisplatin than the NC group. Cell viability was also evaluated using a dye exclusion assay with flow cytometry (b,e,g) after the treatment with 10 μM DDP (for A549 cell line) or 60 μM DDP (for H1299 cell line) or 30 μM DDP (for SK-MES-1 cell line). Representative flow cytometry results showing miR-488 effects on cisplatin induced cell apoptosis in A549 (c) cells, H1299 (f) cells and SK-MES-1 cells (h), the right panels of cell viability refer to cells gated in the C3 quadrants. The results of 3–4 independent experiments are given as the mean ± SD (*P < 0.05).

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