Figure 5: GBS capacity to colonize host organs requires heme-activated respiration metabolism. | Scientific Reports

Figure 5: GBS capacity to colonize host organs requires heme-activated respiration metabolism.

From: Visualization of the role of host heme on the virulence of the heme auxotroph Streptococcus agalactiae

Figure 5

(A) Organ CFU of WT and ΔcydA GBS strains at 13 h post-infection. Mice infected as described in Fig. 2B were euthanized at 13 h post-infection and bacterial burden in dissected organs determined as in (A). Symbols represent data from n = 8 to 10 mice from 3 independent experiments. Two-tailed Mann–Whitney was used to determine P values: heart, P = 0.001; kidney, P = 0.0015; liver, P = 0.97; brain, P = 0.0004. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant (P > 0.05). (B) WT(plux) and ΔcydA(plux) bioluminescence in dissected organs. Representative organs from infected mice were sacrificed 13 h post-infection. Organs were visualized in an IVIS 200 system (acquisition time, 5 min; binning 8).

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