Figure 4: Effects of silencing of Slug on ITGB4 overexpression-induced EMT, cell proliferation and invasion. | Scientific Reports

Figure 4: Effects of silencing of Slug on ITGB4 overexpression-induced EMT, cell proliferation and invasion.

From: Integrin β4 promotes cell invasion and epithelial-mesenchymal transition through the modulation of Slug expression in hepatocellular carcinoma

Figure 4

(A) Bel-7402 and SMMC-7721 cells stably overexpressing ITGB4 were transfected with small interfering RNA (siRNA) against Slug, and cell viability was assessed using the MTT assay, and showed significantly lower rates of cell proliferation compared with scramble-siRNA. (B) and invasive ability of siSlug-transfected cells was decreased as determined by the Transwell assay and quantified after 24 h compared with scramble-siRNA. **P < 0.01. (C,D) The protein expression of ITGB4, Slug and the EMT markers E-cadherin, N-cadherin, and vimentin was determined by western blotting in Bel-7402- ITGB4 and SMMC-7721- ITGB4 cells treated with Slug-siRNA or scramble-siRNA.

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