Figure 1: ECM production by BMSCs on PGA/PLA scaffolds. | Scientific Reports

Figure 1: ECM production by BMSCs on PGA/PLA scaffolds.

From: Repair of osteochondral defects with in vitro engineered cartilage based on autologous bone marrow stromal cells in a swine model

Figure 1

Unwoven PGA fibres were compressed to form a cylindrical-shaped scaffold with porous structure (A–C). BMSCs on the scaffold display round shapes within 24 h and distribute throughout PGA fibres (D–F). Three days after cell seeding, BMSCs started to spread and connect to PGA/PLA fibres with little ECM production (G–I). At 7 days, BMSCs produced enough ECM to wrap around PGA fibres and cover the porous structure (J–L). At 2 weeks, constructs presented an ivory-white appearance and the PGA fibres had been completely covered by abundant ECM (M–O). Scale bar = 50 μm.

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