Figure 2: Gross view and histology of in vitro BMSC cartilage formation. | Scientific Reports

Figure 2: Gross view and histology of in vitro BMSC cartilage formation.

From: Repair of osteochondral defects with in vitro engineered cartilage based on autologous bone marrow stromal cells in a swine model

Figure 2

In vitro chondrogenesis and maturation of BMSCs represented a time-dependent manner. At 2 weeks, constructs started to form cartilage-like tissue at the edge with ECM deposition; at 4 weeks, the constructs basically formed homogeneous, cartilage-like tissue with uniform ECM deposition and un-degraded PGA fibres; at 6 weeks, newly formed cartilage became more mature with typical lacuna structures and less residual PGA; at 8 and 10 weeks, samples became mature, homogeneous cartilage-like tissue with abundant lacuna structures and strongly positive staining for cartilage-specific matrices. Notably, expression of COL I and COL X was detected in all samples at different time points. Scale bar = 100 μm.

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