Figure 1: Enrichment of ECM protein from pancreatic islets and insulinomas. | Scientific Reports

Figure 1: Enrichment of ECM protein from pancreatic islets and insulinomas.

From: Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression

Figure 1

(A) Hematoxylin and eosin staining (upper panel) or collagen staining using Masson’s trichrome (lower panel) of pancreas from 6-week-old wild-type mice and 6-, 9- or 12-week-old RIP1-Tag2 mice. Dashed lines circle islets and insulinomas. Black arrowheads on the upper panels indicate blood vessels; white arrowheads on the lower panels indicate collagen fibers. (B) Anti-collagen I immunoblotting shows that collagen I is not removed during the decellularization of normal and diseased pancreatic islets. Arrows indicate minor soluble fragments of collagen I (<9 kDa) that may be the result of collagenase treatment. (C) The sequential extraction of intracellular components during the decellularization process was monitored by immunoblotting for the cytoskeletal protein, actin; the cytosolic protein, GAPDH; the nuclear histones. The insoluble fraction obtained after decellularization was highly enriched for ECM proteins (see collagen I and laminins panels) and largely depleted for intracellular components.

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