Figure 2: Quantitative proteomic profiling of the ECM of normal pancreatic islets and insulinomas. | Scientific Reports

Figure 2: Quantitative proteomic profiling of the ECM of normal pancreatic islets and insulinomas.

From: Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression

Figure 2

(A) Schematic illustration of the iTRAQ-based quantitative proteomic pipeline used in this study. Fragmentation of MS-selected iTRAQ-tagged peptides in MS/MS yields both peptide identity and relative quantities as it generates low molecular mass-reporter ions with 4 distinct masses (114, 115, 116, and117 - represented by squares). (B) Pie charts represent the distribution of ECM and ECM-associated proteins within the different matrisome categories in replicate A (left panel) and B (right panel). (C) Venn diagram represents the overlap between ECM and ECM-associated proteins detected in the two biological replicates. The 120 ECM and ECM-associated proteins detected in both replicates are listed in Table 1.

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