Figure 5: Effects of FAS2 RNAi on the duration of diapause preparation and incidence of diapause in adult female C. bowringi.

The time elapsed before adult female C. bowringi dug into soil to commence diapause was observed at 12 h intervals during the 7 days after dsRNA injection. Values are means ± SD based on three independent biological replicates. dsGFP n = 46, 49, 51; dsFAS2 n = 43, 46, 46. Log-rank (Mantel-Cox) test in Kaplan-Meier survival analysis was used to show the variation in the duration of diapause preparation between dsGFP (total n = 146) and dsFAS2 (total n = 135) groups.