Table 1 Primer sequences for qRT-PCR and dsRNA synthesis.
Genes | Forward primers (5′-3′) | Reverse primers (5′-3′) | E (%) | R2 |
|---|---|---|---|---|
qRT-PCR | ||||
 RPL19 | gtaatgcgatgcggcaagaa | gagtgcaccgctacaggttt | 102.2 | 0.998 |
 FAS1 | ggccaacgagttatgggtat | agggcataaacagtcgttcc | 90.3 | 0.992 |
 FAS2 | cactgaactctgccacgtct | cgcgtttgccactagaatcg | 92.5 | 0.991 |
 Hsp21 | acgtcaggtcctggagatca | tgaactgctgaacgtcgaga | 98.0 | 0.990 |
 Hsp23 | tgtactgagaccgctgagga | gtagcgaggcctgtttggta | 90.7 | 0.988 |
 Hsp70 | tgactttcgatctggacgcc | gtcgatatcctgctgcgaca | 93.8 | 0.990 |
 SOD | aagctgggacatatagcgcc | gcctaggtctccaacgtgtc | 98.2 | 0.996 |
 GST | tcgaaatacgccaaagggca | tctcagtggttccaacaggc | 104.1 | 0.994 |
 Met | caattgctcaacacccagcc | ccttcgttgagcgacagtct | 98.2 | 0.998 |
RNAi | ||||
 GFP | gcgtaatacgactcactataggtggtcccaattctcgtggaac | gcgtaatacgactcactataggcttgaagttgaccttgatgcc | n.a. | n.a. |
 FAS2 | gcgtaatacgactcactataggaagaacacaaccttccacgg | gcgtaatacgactcactataggccgcttctggaagtgagaac | n.a. | n.a. |
 Met | gcgtaatacgactcactataggatgattgaggaagtgtcggg | gcgtaatacgactcactatagggattctcgtggtggaccagt | n.a. | n.a. |
