Figure 3: TRPM7 and MagT1 in LoVo-S and LoVo-R.

LoVo cells were harvested every 48 h for 6 days and lysed. Part of the sample (100 μg) was run on a 10% SDS-PAGE and probed with anti-MagT1 antibodies. The same amounts of cell lysates were separated on a 8% SDS-PAGE and utilized for Western blot using anti-TRPM7 antibodies. Blots were processed in parallel. Actin was used as a control of loading. Full-length blots and quantification are presented in Supplementary Figure S3.