Figure 4: Effect of TRPM7 silencing on LoVo-S proliferation.

(a) Cells were treated with the TRPM7 channel blocker 2-APB (50 μM) and counted every 24 h for 3 days. Data are express as % of the control and refer to three separate experiments in triplicate ± standard deviation. (b) LoVo-S were transfected with a siRNA against TRPM7 or with a non-silencing siRNA. After 24 h RNA was extracted and Real-Time PCR performed using primers designed on TRPM7 sequence. (c) Western blot was performed on cell extracts of LoVo-S 24, 48 and 72 h after exposure to siRNA. Actin was used as a control of loading. Full-length blots and quantification are presented in Supplementary Figure S4C. (d) LoVo-S treated as described above were counted every 24 h. Data are expressed as total cell number and refer to three separate experiments in triplicate ± standard deviation.