Figure 6: MagT1 silencing and LoVo-R proliferation. | Scientific Reports

Figure 6: MagT1 silencing and LoVo-R proliferation.

From: The different expression of TRPM7 and MagT1 impacts on the proliferation of colon carcinoma cells sensitive or resistant to doxorubicin

Figure 6

LoVo-R were transfected with a siRNA against MagT1 or with a non-silencing siRNA. (a) After 24 h Real-Time PCR was performed using primers designed on MagT1 sequence. (b) Western blot was performed on cell extracts of LoVo-R 24, 48 and 72 h after transfection with siRNA against MagT1. Actin was used as a control of loading. Full-length blots and quantification are presented in Supplementary Figure S6B. (c) LoVo-R were counted every 24 h. Data are expressed as total cell number and refer to three separate experiments in triplicate ± standard deviation.

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