Table 4 Primers used for SNPs genotyping (labeled with G) and gene expression detection (labeled with RT).
|  |  | Primer sequence (5′–3′) | Tm (°C) |
|---|---|---|---|
nLvALF2 (G) | Forward | ACTAACCCTTTCGCTCCCACCCAC | 56 |
| Â | Reverse | TATTGGATGAGGTATCAACATTCGC | Â |
nLvALF2 (RT) | Forward | GGCAACTGTATTTCAGGGGTCG | 54 |
| Â | Reverse | CTGCGTGTCGTTCTTCTTCG | Â |
TRAF6 (G & RT) | Forward | CTGACCCTTTAGTGGACGCAT | 57 |
| Â | Reverse | AGGTTCCTGTGCTGGGTTGA | Â |
Cu/Zn SOD (G & RT) | Forward | TTGGACTTCCACCGCCAC | 57 |
| Â | Reverse | TCCAGTCCAGGGAAATGTGC | Â |
18 S rRNA | Forward | TATACGCTAGTGGAGCTGGAA | 55 |
| Â | Reverse | GGGGAGGTAGTGACGAAAAAT | Â |
