Figure 6: B16shR-SOCS1 cells microenvironment and protective role of CD8+ T lymphocytes. | Scientific Reports

Figure 6: B16shR-SOCS1 cells microenvironment and protective role of CD8+ T lymphocytes.

From: SOCS1 favors the epithelial-mesenchymal transition in melanoma, promotes tumor progression and prevents antitumor immunity by PD-L1 expression

Figure 6

(A) SOCS1-silenced cells enhance cellular infiltrate in the s.c. tumor microenvironment (black arrows); T-CD8 and NK.1.1 cells in tumor infiltrate are shown by FACScan flow cytometer with median fluorescence intensity (MFI); (B) SOCS1-silencing down-regulates MMP2, MMP9 and CD10 expression; (C) T-CD4+-KO and T-CD8+-KO mice were submitted to prophylactic treatment with SOCS1-silenced cells and were rechallenged with WT B16F10-Nex2 cells. The treatment was not effective in T-CD8+-KO mice. Student-t test (*p < 0.05; **p < 0.01). A representative blot from three independent experiments is shown for each panel. *Results were normalized by β-actin.

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