Figure 9: Immunohistochemistry of (II) lung tissues, from B16shR-SOCS1 prophylactic treatment. | Scientific Reports

Figure 9: Immunohistochemistry of (II) lung tissues, from B16shR-SOCS1 prophylactic treatment.

From: SOCS1 favors the epithelial-mesenchymal transition in melanoma, promotes tumor progression and prevents antitumor immunity by PD-L1 expression

Figure 9

Left panels, animal tissues challenged s.c. with murine melanoma cells B16F10-Nex2 and intravenously rechallenged with the same WT tumor cells; right panels, the same protocol with primary s.c. challenge with B16shR-SOCS1 cells. (A,B) CD3 staining; (C,D) CD4 staining; (E,F) CD8 staining (G,H) CD11b staining; (I, J) CD4+FoxP3+ T cell staining and (K, L) PD-L1 staining. Counterstaining: hematoxylin; magnification, × 200. Graphs represent optical densities of 3,3′-Diaminobenzidine (DAB) staining of 10 different fields from 3 different mice. Student t-test (**p = 0.027; ***p < 0.001).

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