Figure 1: Interaction of MYPT1 with PRMT5. | Scientific Reports

Figure 1: Interaction of MYPT1 with PRMT5.

From: Myosin phosphatase and RhoA-activated kinase modulate arginine methylation by the regulation of protein arginine methyltransferase 5 in hepatocellular carcinoma cells

Figure 1

(A) Immunoprecipitations were carried out using anti-MYPT11-296 and anti-PRMT5 antibodies as well as nonimmune rabbit serum as negative control. Immunoprecipitates and HepG2 total lysate were analysed by Western blots using antibodies specific for MYPT1 and PRMT5. (B) SPR analysis of the interaction of MYPT1 with PRMT5. Full-length GST-MYPT11-1004 was immobilized on anti-GST coupled CM5 sensor chip. FT-PRMT5 was injected over the surfaces in the indicated concentrations. The interaction was monitored using Biacore 3000. The association constant (Ka) value was indicated in the figure.

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