Figure 4: Microarray analysis of MYPT1-silenced HepG2 cells. | Scientific Reports

Figure 4: Microarray analysis of MYPT1-silenced HepG2 cells.

From: Myosin phosphatase and RhoA-activated kinase modulate arginine methylation by the regulation of protein arginine methyltransferase 5 in hepatocellular carcinoma cells

Figure 4

(A) Heat map of genes related to MYPT1 silencing in HepG2 cells by microarray analysis. The color code for the signal strength is shown in the box at the bottom in which induced genes are indicated by red and repressed genes are indicated by blue. (B) The onthology of the related genes and their classification by GO terms. Detection of retinoblastoma protein (C) and c-Myc (D) protein expression changes due to MYPT1 silencing from HepG2 whole cell lysates by Western blot. Protein levels were quantified by densitometry normalized to α-tubulin or GAPDH expression level. Samples derived from the same experiment and processed in parallel. RT-PCR analysis of MYPT1, PRMT5 and RAP1A mRNA levels in non-specific siRNA treated and MYPT1 silenced HepG2 cells (E). GAPDH was used as an invariant gene. Values are mean ± SEM from three independent experiments; *p < 0.05, **p < 0.01 by student t-test.

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