Figure 5: Protein expression profile of HCC and other cancer cell lines. | Scientific Reports

Figure 5: Protein expression profile of HCC and other cancer cell lines.

From: Myosin phosphatase and RhoA-activated kinase modulate arginine methylation by the regulation of protein arginine methyltransferase 5 in hepatocellular carcinoma cells

Figure 5

Reverse phase protein microarray analysis was conducted to study the changes in protein expression and posttranslational modification of PRMT5 (A,B), MYPT1 (C,D) and histone H2A (E,F) proteins in normal and tumor human cell lysates. Human HCC samples (n = 20) were grouped based on their clinically verified stage (middle grey columns) or grade (dark grey columns) classification of tumor. Average of HCC samples irrespectively of grouping and other types of metastatic liver cancer tissues are shown next to each other (light grey columns). Protein microarray contains 15 cancer cell lines and normal tissue lysate of controls of corresponding organs in triplicates (black columns). Value of 1 means average of relative expression, phosphorylation or symmetrical dimethylation of the given protein or residue to the corresponding non-tumor samples. Blots were processed under the same experimental procedure. Datas are mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 by student t-test.

Back to article page