Figure 1: Experimental workflow. | Scientific Reports

Figure 1: Experimental workflow.

From: Differential Effects of Vitamins A and D on the Transcriptional Landscape of Human Monocytes during Infection

Figure 1

Human monocytes were isolated from fresh whole blood and purity of the cells was analyzed by flow cytometry. Upper scatterplot: Forward scatter (FSC) and side scatter (SSC) measurement. Lower scatterplot: Fluorescence intensities of cells stained with FITC-conjugated CD14 antibody and APC-conjugated CD16 antibody. Monocytes were then pre-incubated with vitamin A (atRA) or vitamin D, followed by stimulation with heat-killed A. fumigatus, C. albicans or E. coli for 6 h. Poly-(A) RNA was isolated from the monocytes and subjected to RNA sequencing.

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