Figure 3: Heatmap of K-means clustering of DEGs and subsequent GO enrichment analysis. | Scientific Reports

Figure 3: Heatmap of K-means clustering of DEGs and subsequent GO enrichment analysis.

From: Differential Effects of Vitamins A and D on the Transcriptional Landscape of Human Monocytes during Infection

Figure 3

K-means clustering was performed on variance-stabilized read counts to build a heatmap for the 6076 differentially expressed protein-coding genes (adjusted p-value < 0.05). A priori, the model-based optimal number of K = 18 was determined. The clustering of the rows is based on euclidean distance. The colors in the map represent row-scaled expression levels: blue indicates the lowest expression, white indicates intermediate expression, and red indicates the highest expression. Selected clusters were analysed with regard to their biological function by GO enrichment analysis. Most enriched GO categories are shown for representative groups of clusters displaying their fold enrichment.

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