Figure 1: Diagram of the method used for quantifying activity of specific transporter species.
From: Quantifying the relative contributions of different solute carriers to aggregate substrate transport
The left panel is a schematic of the steps taken in the current study to establish and verify the method for amino acid transport in Xenopus laevis oocytes. AAT refers to Solute Carrier amino acid transporters for L-leucine and L-phenylalanine. The source for the mRNA expression data was Xenbase as described in Methods and Results. Uptake assay protocols are described in Methods. Defined uptake buffers are described in Methods, Results, and Fig. 2. The input parameters for the calculations are given in Table 1. The rationale for the specific equations used to construct the current model are given in the methods. The right panel is a generalized series of steps that could be applied to any system where the kinetics of each active enzyme species can be represented by the Hill equation.
