Figure 2: HD-DCs do not suppress DNBS in IL-4Rα−/− mice. | Scientific Reports

Figure 2: HD-DCs do not suppress DNBS in IL-4Rα−/− mice.

From: Suppression of colitis by adoptive transfer of helminth antigen-treated dendritic cells requires interleukin-4 receptor-α signaling

Figure 2

In-house bred wild-type (WT) or IL-4Rα−/− on a Balb/c background were given 106 HD-DC ip. 48 h prior to DNBS (3 mg, i.r.) challenge. Mice were necropsied and assessed 72 h later via (A) macroscopic damage score and (B) histological damage score; (C) representative micrographs of H&E stained cross-sections of colonic tissue: L; lumen; M: muscle; scale bar 300 μm. Splenocytes were isolated (5 × 106), stimulated with conA (2 μg/mL, 48 h) and supernatants examined for levels of IL-4 (D) and IL-10 (E) by ELISA. Data are shown as mean ± SEM; A–C are representative from one of two independent experiments, (*p ≤ 0.05 vs. DNBS group, Kruskal-Wallis with Dunn’s post-test, n = 3–6 mice/group) and D,E are pooled from two independent experiments (n = 7–9; two-tailed unpaired Student’s t-test).

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