Figure 5: IL-10 KO HD-DCs cannot suppress colitis. | Scientific Reports

Figure 5: IL-10 KO HD-DCs cannot suppress colitis.

From: Suppression of colitis by adoptive transfer of helminth antigen-treated dendritic cells requires interleukin-4 receptor-α signaling

Figure 5

Balb/c mice were given 106 wild-type or IL-10−/− HD-DC i.p. 48 h prior to DNBS (3 mg, i.r.) challenge. Mice were necropsied and assessed 72 h later via (A) macroscopic damage score and (B) histological damage score using Kruskal-Wallis with Dunn’s post-test. (C) Representative micrographs of H&E stained cross-sections of colonic tissue: L; lumen; M: muscle; scale bar 300 μm. Splenocytes were isolated (5 × 106), stimulated with conA (2 μg/mL, 48 h) and supernatants examined for levels of IL-4 (D) and IL-10 (E) by ELISA, and analyzed by one-way ANOVA with Tukey’s post-test. Data are shown as mean ± SEM and are pooled from two independent experiments; n = 6–10 mice/group; *p ≤ 0.05, **p ≤ 0.01 compared to DNBS, # compared to control, @ compared to IL-10 KO HD-DCs.

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