Figure 2: Immunohistochemical (IHC) staining of EGFRK721me1 in SCCHN tissues and correlation with WHSC1L1 staining. | Scientific Reports

Figure 2: Immunohistochemical (IHC) staining of EGFRK721me1 in SCCHN tissues and correlation with WHSC1L1 staining.

From: WHSC1L1-mediated EGFR mono-methylation enhances the cytoplasmic and nuclear oncogenic activity of EGFR in head and neck cancer

Figure 2

(a) (i) Representative examples of EGFRK721me1 IHC staining (+1, +2, +3 scores) in SCCHN tissue sections. Staining of EGFRK721me1 is mostly observed in the nucleus, with mild staining in the cytoplasm (40x). (ii) Representative example of EGFRK721me1 staining in normal squamous epithelium. Staining was mostly observed in the cells of the basal layer of the epithelium, corresponding to the staining of WHSC1L1 (20x). (b) Histogram of immunohistochemistry scores of EGFRK721me1 in normal, dysplastic epithelium and SCCHN tissue sections. The ratio of +2/+3 EGFRK721me1-positive staining samples was statistically significantly higher in SCCHN tissues compared to normal and dysplastic squamous epithelium (Cochran-Armitage test, *p < 0.001). (c) Representative examples of EGFRK721me1 and concordant WHSC1L1 staining in SCCHN sections from 3 different patients (40x). A table with the cumulative IHC score results of SCCHN sections (N = 127) staining for WHSC1L1 and EGFRK721me1 is also shown (Cochran-Armitage test, p = 0.0059).

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