Figure 1

(a) Screening system outline. EST: Expressed Sequence Tag, *X-gal staining was performed in detail only on bone in the second screening. (b) Biomechanical strength analysis and bone morphometric analysis results from male trap lines in the second screening. Lines inside and outside the frame indicate those with and without an observed difference in the second screening, respectively. Selection reasons in the first screening are indicated by colour. Bold and italic fonts indicate positive and negative X-gal staining in the second screening, respectively. *A positive line in the growth curve, §A positive line in the biochemical blood test (Supplementary Table S2).