Figure 3: First and second screening results of Ayu21-W268. | Scientific Reports

Figure 3: First and second screening results of Ayu21-W268.

From: Development of an efficient screening system to identify novel bone metabolism-related genes using the exchangeable gene trap mutagenesis mouse models

Figure 3

(a) Left: X-gal staining results in the first screening of the Ayu21-W268 (Lamin B receptor (Lbr) gene trap mouse line) in the EGTC. (1) ear, (2) cerebral base, (3) eye, (4) brain, (5) tongue-lung, oesophagus, (6) heart, (7) lung (slice), (8) thymus, (9) liver, (10) spleen, pancreas, (11) adrenal gland, (12) kidney, (13) muscle, (14) stomach, (15) duodenum, (16) rectum, (17) genital tract, adipose tissue, (18) bladder, (19) brown adipose tissue, (20) femur. Bar: 20 mm; left: heterozygote, right: negative control. Right: X-gal femoral staining in the first screening. Bar: 4 mm (http://egtc.jp). (b) Micro-computed tomography (μCT) image in the second screening. Left: CT image, right: bone mineral density (BMD) image. Colour scale indicates BMD; 100 mg/cm3 (blue) and 1550 mg/cm3 (yellow). Bar: 1 mm. (c) Graphs showing the results of bone morphometric analysis. Tb.BMD: trabecular bone mineral density, Tb.BMC: trabecular bone mineral content, Ct.BMD: cortical bone mineral density, Ct.BMC: cortical bone mineral content, Ct.V: cortical bone volume, Ct.Th: cortical bone thickness, Ex.Ln.Le: external line length, BV: trabecular bone volume, Tb.Th: trabecular bone thickness, Tb.N: trabecular bone number, V*m.space: marrow space star volume. (d) Three-point bending test results on femurs of Lbr+/+ and LbrGT/GT mice in the second screening. M.load: maximum load, M.stress: maximum stress, M.dsp: maximum displacement, M.work: maximum work, E.M: elastic modulus. Black points show each value, red points show mean of Lbr+/+ and LbrGT/GT mice, and red bars show 1.0 and 2.0 SD of Lbr+/+ mice.

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