Figure 4: First and second screening results of Ayu21-T269 and Ayu21-W203. | Scientific Reports

Figure 4: First and second screening results of Ayu21-T269 and Ayu21-W203.

From: Development of an efficient screening system to identify novel bone metabolism-related genes using the exchangeable gene trap mutagenesis mouse models

Figure 4

(a) Femur X-gal staining results in the first screening of Ayu21-T269 (Neural precursor cell expressed, developmentally down-regulated gene 4 (Nedd4) gene trap mouse line) in the EGTC database. Left: negative control, right: heterozygote. Bar: 2 mm (http://egtc.jp). (b) Micro-computed tomography (μCT) image in the second screening. Left: CT image, right: BMD image. Colour scale indicates BMD; 100 mg/cm3 (blue) and 1550 mg/cm3 (yellow). Bar: 1 mm. (c) Graphs showing the results of bone morphometric analysis. (d) Three-point bending test results on femurs of Nedd4+/+ and Nedd4GT/+ mice in the second screening. (e) Femur X-gal staining results in the second screening of Ayu21-W203 (Novel gene trap mouse line). Left: negative control, right: homozygote. Bar: 1 mm. (f) μCT image in the second screening. Left: CT image, right: BMD image. Colour scale indicates BMD; 100 mg/cm3 (blue) and 1550 mg/cm3 (yellow). Bar: 1 mm. (g) Graphs showing the results of BMA in the second screening. (h) Three-point bending test results on femurs of Novel+/+ and NovelGT/GT mice in the second screening. Black points show each value, red points show mean, and red bars show 1.0 and 2.0 SD.

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