Figure 5: Mouse line analyses of Ayu21-W268 and Ayu21-T269 trap lines after screening. | Scientific Reports

Figure 5: Mouse line analyses of Ayu21-W268 and Ayu21-T269 trap lines after screening.

From: Development of an efficient screening system to identify novel bone metabolism-related genes using the exchangeable gene trap mutagenesis mouse models

Figure 5

(a) Upper: Lbr+/+ and LbrGT/GT mice at 4 weeks old. The LbrGT/GT mouse shows sparseness of hair, presence of scales most predominantly on the tail, and decreased body size. Bar:10 mm. Lower: photo and micro-computed tomography (μ-CT) images of hind paw. The LbrGT/GT mouse shows syndactyly characterized by soft tissue fusion between digits three and four (arrows). Bar: 1 mm. (b) Three dimensional structural indices of femurs of Lbr+/+ and LbrGT/GT mice at 4 weeks old in bone morphometric analysis. (c) Three-point bending test results on femurs of Lbr+/+ and LbrGT/GT mice at 4 weeks old. (d) Real-time PCR analysis of bone metabolism-related genes of femurs of Lbr+/+ and LbrGT/GT mice at 4 weeks old. (e) Transparent skeletal specimen of Nedd4+/+ and Nedd4GT/GT mice at 4 weeks old. Nedd4GT/GT mice show decreased body size. Bar: 10 mm. (f) Three dimensional structural indices of femurs of Nedd4+/+ and Nedd4GT/GT mice at 4 weeks old in bone morphometric analysis. (g) Three-point bending test results on femurs of Nedd4+/+ and Nedd4GT/GT mice at 4 weeks old. (h) Real-time PCR analysis of bone metabolism-related genes of femurs of Nedd4+/+ and Nedd4GT/GT mice at 4 weeks old. Relative expression levels were normalized to the ratio of an internal β-actin standard and wild-type mouse gene expression levels. Results are mean ± SD (n = 5). Student’s t-tests: *P < 0.05, **P < 0.01.

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