Figure 5: The mRNA levels of selected proteins analyzed by using qRT-PCR. | Scientific Reports

Figure 5: The mRNA levels of selected proteins analyzed by using qRT-PCR.

From: Proteome Response of Chicken Embryo Fibroblast Cells to Recombinant H5N1 Avian Influenza Viruses with Different Neuraminidase Stalk Lengths

Figure 5

Groups of CEF cells (n = 3) were infected with 106 EID50 of the indicated virus or with PBS as a control. The cells were collected at 12, 24 and 36 hpi for mRNA extraction. qRT-PCR was performed to validate eight genes and the mRNA levels of selected genes at each time point were analyzed with chicken histone H5 gene as reference for expression normalization.

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