Figure 7: Subcellular distribution of cytoskeleton proteins vimentin and tubulin in H5N1 infected cells by IFA.

NP protein was recognized with mice antiserum to H5N1-NP followed by FITC conjugated IgG (green). Vimentin and β-tubulin in H5N1 infected cells were detected using mouse mAbs followed by CY3-conjugated IgG (red). The nucleus was stained with DAPI (blue). The triple stained cells were observed by a Zeiss LSM510 laser confocal microscopy. The arrowheads represent uninfected cells, and arrows indicate H5N1 infected cells. (A) The subcellular location of vimentin filaments in H5N1 infected CEF cells using double staining IFA at 6 hpi. (B) The filamentous vimentin was considerably broken down in rNA-wt infected cells at 12 hpi. (C) The array of radial tubulin microtubules disappeared in the H5N1 infected CEF cells.