Figure 1: DOPAL effect on vesicle trafficking in aS overexpressing cells.
From: DOPAL derived alpha-synuclein oligomers impair synaptic vesicles physiological function
(a) Confocal images of BE(2)-M17 cells transfected with mCherry or aS-mCherry and synapto-pHluorin. (b) Normalized fluorescence intensity/number of vesicles cumulative graphs for mCherry and aS. Black and red traces refer to untreated cells, and cells treated with DOPAL 100 μM for 24 hours. (c) Histograms express the ratio between fixed (see Methods for details) and total vesicles over a certain period of time, i.e. the number of vesicles showing a high fluorescent value for the whole time span of the measure, for mCherry or aS-mCherry overexpressing cells untreated or treated with 100 μM for 24 hours. (d) Average distance covered by vesicles in cells overexpressing mCherry or aS-mCherry at time 0 and after 100 μM DOPAL treatment at 24 hours. White and black stand for control cells and cells treated with 100 μM DOPAL for 24 hours. Bars represent mean ± SEM from n = 25–30 cells from at least three independent experiments. Asterisks indicate statistical significance by two-way ANOVA (**p < 0.01, ***p < 0.001).
