Figure 3: aS-DOPAL oligomers are found in neuronal cell models upon DOPAL treatment.
From: DOPAL derived alpha-synuclein oligomers impair synaptic vesicles physiological function
(a) Western blot analysis of BE(2)-M17 cells overexpressing aS-mCherry and treated with 100 μM DOPAL for 1 hour, 18 hours or 24 hours. aS antibody detected aS monomers and oligomers (left) and after the pull-down with ABPA resin (right) the band showing monomeric aS modified by DOPAL is larger than the control after 18 or 24 hours treatment. (b) Western blot analysis of HEK293 cells treated with 100 μM DOPAL showing DOPAL-induced aS oligomers at high molecular weight (left) and the pull-down with ABPA resin (right) show that DOPAL-modified aS monomer is accumulated upon treatment. (c) Western blot of primary cortical neurons upon 20 μM and 50 μM DOPAL treatment show monomeric aS and aS-DOPAL oligomers accumulation. (d) ABPA resin pull-down from neuronal lysate of control and treated samples suggested that the accumulated monomeric aS is modified by DOPAL.
