Figure 7: Effect of down-regulation of tight junction RNA transcripts on outflow facility ex vivo.
(a) ‘Cello’ plots showing the individual values and statistical distribution of outflow facility at 8 mmHg (Cr) for eyes treated with either non-targeting (NT) siRNA or a combination of ZO-1 and tricellulin targeting (T) siRNA. Each individual point represents a single eye, with error bars showing the 95% confidence intervals on Cr arising from the regression analysis. For each condition, the predicted log-normal distribution is shown, with the thick central white band showing the geometric mean and the thinner white bands showing two geometric standard deviations from the mean. The shaded central region indicates the 95% confidence interval on the mean. (b) Paired facility plot: each data point represents one pair of eyes, with Cr for the treated T siRNA eye on the Y-axis and the Cr for contralateral control NT-siRNA eye on the X-axis. The red line shows the average difference between contralateral eyes, with its confidence interval in grey, whilst the blue line represents the case of identical facility between contralateral eyes, corresponding to no effect due to T siRNA. All data points are above the blue unity line, indicating that the facility was higher in the treated eyes compared to the controls; n = 7, p = 0.006. Inner blue ellipses show the 95% confidence intervals on Cr arising from the regression analysis, whilst the green outer ellipses show additional uncertainty due variability between contralateral eyes, estimated from 10 pairs of C57BL/6J eyes perfused only with glucose supplemented PBS36.
