Figure 3: MiR-124 regulates the transcription factor Sp1. | Scientific Reports

Figure 3: MiR-124 regulates the transcription factor Sp1.

From: MiR-124 inhibits the migration and invasion of human hepatocellular carcinoma cells by suppressing integrin αV expression

Figure 3

(A) The sequences of human miR-124-1, 2, 3 genomic loci (a). Framed part indicates the mature sequence. Four online algorithms were used to predicate miR-124 targets (b). (B) Network of predicated miR-124-targeted transcription factors and the association with ITGAV. The red rectangle indicates miR-124, the yellow diamond indicates ITGAV gene, and the blue circles indicate the miR-124-targeted TF mRNAs. The TFs linking to ITGAV have specific binding sites on ITGAV promoter. (C) Sp1 has two conserved miR-124 binding sites within 3′UTR. (D) The schematic of miR-124 binding site 1 mutation (M1) of Sp1 3′UTR. (E) Reporter luciferase activities were measured in HEK-293T (upper) and HeLa (lower) cells. Data are representative of three independent experiments. **p < 0.01.

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