Figure 3: Yeast Tom22 and plant Tom9 do not complement for lack of ATOM14. | Scientific Reports

Figure 3: Yeast Tom22 and plant Tom9 do not complement for lack of ATOM14.

From: Mitochondrial protein import - Functional analysis of the highly diverged Tom22 orthologue of Trypanosoma brucei

Figure 3

(A) Growth curve of the uninduced (−Tet) and induced (+Tet) ATOM14 3′UTR RNAi cell line. Right panel, immunoblot probed for ATOM14 and elongation factor 1a (EF1a) which serves as a loading control. (B) ATOM14 3′UTR RNAi cell line ectopically expressing wildtype ATOM14 (light grey) under Tet control. The addition of Tet induces simultaneous upregulation of the ectopic copy (exo mRNA) of the ATOM14 gene and downregulation of the mRNA transcribed from the endogenous ATOM14 gene (endo mRNA), respectively. (C) As in (B) but the complementation was performed using the yeast Tom22 gene (medium grey). Right panel, immunoblot of total, pellet and supernatant fractions of a digitonin fractionation using the same complemented cell line probed with an antiserum specific for yeast Tom22. (D) As in (B) but the complementation was done using the plant Tom9 gene (dark grey). The ethidium-stained rRNAs below the Northern blots of (B,C and D) serve as loading controls. The position of the membrane spanning domain in the different Tom22 orthologues is indicated.

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