Figure 4: The cytosolic domain of ATOM14 is not essential.

(A) Growth curve of an ATOM14 3′UTR RNAi cell line ectopically expressing an ATOM14 (light grey) variant lacking the cytsosolic domain. (B) As in (A) but complementation was done using an ATOM14 variant (light grey) whose cytosolic domain was replaced by the corresponding domain of yeast Tom22 (medium grey). (C) As in (A and B) but complementation was done using an ATOM14 variant (light grey) whose cytosolic domain was replaced by the corresponding domain of plant Tom9 (dark grey). In all three cases addition of Tet induces simultaneous upregulation of the ectopic copy (exo mRNA) of the corresponding ATOM14 variant gene and downregulation of the mRNA transcribed from the endogenous ATOM14 gene (endo mRNA), respectively. The ethidiumbromide-stained rRNAs below the Northern blots of (A,B and C) serve as loading controls.